Investigating the effects of Sea anemone (Stichodactyla haddoni) mucus on prostate cancer PC3 cell line

Background & Aim: Prostate cancer (PCa) is one of the most common cancers of the male urinary system and is also the second leading cause of cancer deaths in men. In the present study, the effects of Stichodactyla haddoni on the viability of prostate cancer cells (PC3) were investigated. Methods: In the semi experimental study, sea anemones were sampled at random using an identification key. Then concentration of crude mucus obtained from S. haddoni sea anemone was carried out by acetone. The PC3 cells treated with different concentrations of crude mucus (20, 40, 60, 80, 100 and 120 µg/ml) and Doxorubicin anticancer drug (0.06, 0.25 and 0.5 μg/ml) as a positive control for 24 and 48h. The effect of treatments on cell viability was evaluated using MTT assay. The IC50 of crude mucus was calculated after 24 and 48 h. Results: The inhibitory effects of the crude mucus of S. haddoni was increased with the growth of PC3 cells with escalating the dosage and treatment time (24 and 48h). The Doxorubicin anticancer drug induced the highest cytotoxicity at different concentrations, which was similar to the concentration of 100 and 120 µg/ml of crude mucus. Conclusion: The crude mucus of S. haddoni has cytotoxic and growth inhibitory effects on PC3 cancer cells, therefore, it can be considered as an appropriate candidate for the development of anticancer drugs.